BD-PE0617-NTRK3 (TrkC) (14B6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NTRK3 (TrkC) (14B6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0617

产品名称

NTRK3 (TrkC) (14B6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NTRK3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human NTRK3 expression vector

稀释度

WB 1:1000 IHC 1:50 IF 1:100

预测分子量

94.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

期货

2周

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NTRK3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NTRK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunofluorescent staining of HT29 cells using anti-NTRK3 mouse monoclonal antibody (BD-PE0617).

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

Anti-NTRK3 mouse monoclonal antibody (BD-PE0617) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NTRK3 .

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-NTRK3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0617, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTRK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK3.