BD-PE0597-RGS10 (7T5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RGS10 (7T5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0597

产品名称

RGS10 (7T5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RGS10

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RGS10 (NP_001005339) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:2000

预测分子量

21kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RGS10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0597) (1:2000)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RGS10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0597) (1:2000)

Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-RGS10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0597) (1:2000)

Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-RGS10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0597) (1:2000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS10(Cat# BD-PE0597). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.