BD-PE0577-GATA3 (6Q7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GATA3 (6Q7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0577

产品名称

GATA3 (6Q7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GATA3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.

稀释度

IHC 1:200

预测分子量

47.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0577) (1:200)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0577) (1:200)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0577) (1:200)

Flow cytometric Analysis of MCF-7 cells, using anti-GATA3 antibody (BD-PE0577), (Red), compared to a nonspecific negative control antibody , (Blue).The right is HUVEC cells as negtive control (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0577) (1:200)

Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (BD-PE0577) (Left). The right is negative control. (1:2000)

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody (1:200).