Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP16 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 137-466 of human USP16 (NP_001001992) were separated by SDS-PAGE and immunoblotted with anti-USP16.