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POR (Cytochrome P450 Reductase) (13P5) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0545
产品名称
POR (Cytochrome P450 Reductase) (13P5) Mouse Monoclonal antibody
类别
常规抗体
基因名称
POR
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human POR (NP_000932) produced in HEK293T cell.
稀释度
WB 1:500, IHC 1:50, FCM 1:100
预测分子量
76.9kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POR antibody (BD-PE0545), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0545)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-POR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0545)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-POR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0545)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0545)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POR. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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