BD-PE0535-ABCB1 (4G5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ABCB1 (4G5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0535

产品名称

ABCB1 (4G5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ABCB1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.

稀释度

WB 1:2000, IHC 1:2000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0535) (1:2000)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0535) (1:2000)

Flow cytometric Analysis of permeabilized Hek293T cells, using anti-ABCB1 antibody (BD-PE0535), (Red), compared to negative control (PBS), (Blue) (1:100).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0535) (1:2000)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0535) (1:2000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCB1 (Cat# BD-PE0535)(1:2000).