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PDE1B (5E18) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0525
产品名称
PDE1B (5E18) Mouse Monoclonal antibody
类别
常规抗体
基因名称
PDE1B
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
稀释度
WB 1:2000, IF 1:100
预测分子量
61.2kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Anti-PDE1B mouse monoclonal antibody (BD-PE0525) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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