BD-PE0524-PDE1B (5H19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PDE1B (5H19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0524

产品名称

PDE1B (5H19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PDE1B

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.

稀释度

WB 1:500~2000, IF 1:100, FCM 1:100

预测分子量

61.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]). [supplied by OMIM]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody (BD-PE0524), and then analyzed by flow cytometry.

Anti-PDE1B mouse monoclonal antibody (BD-PE0524) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.