BD-PE0490-GSTT2 (Glutathione S Transferase theta 2) (14D10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GSTT2 (Glutathione S Transferase theta 2) (14D10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0490

产品名称

GSTT2 (Glutathione S Transferase theta 2) (14D10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GSTT2

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GSTT2 (NP_000845) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IF 1:100, FCM 1:100

预测分子量

27.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSTT2 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSTT2 antibody (BD-PE0490), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-GSTT2 antibody (BD-PE0490), (Red), compared to a nonspecific negative control antibody , (Blue).

Anti-GSTT2 mouse monoclonal antibody (BD-PE0490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSTT2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTT2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.