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aFGF (FGF1) (14N13) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0477
产品名称
aFGF (FGF1) (14N13) Mouse Monoclonal antibody
类别
常规抗体
基因名称
FGF1
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.
稀释度
WB 1:5000~50000, IF 1:100
预测分子量
15.8kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

aFGF antibody ( 4D2 ) at 1:50000 dilution + recombinant Human aFGF

Anti-AFGF mouse monoclonal antibody (BD-PE0477) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFGF .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFGF (Cat# BD-PE0477). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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