BD-PE0475-DHFR (Dihydrofolate reductase) (19T1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DHFR (Dihydrofolate reductase) (19T1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0475

产品名称

DHFR (Dihydrofolate reductase) (19T1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DHFR

推荐应用

反应种属

Human,Monkey,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

稀释度

WB 1:2000 IHC 1:50 IF 1:100

预测分子量

21.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0475, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0475, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0475, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0475, Dilution 1:50)

Anti-DHFR mouse monoclonal antibody (BD-PE0475) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFR .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.