BD-PE0471-DCK (5F4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DCK (5F4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0471

产品名称

DCK (5F4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DCK

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human DCK (NP_000779) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, FCM 1:100

预测分子量

30.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DCK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0471)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0471)

Flow cytometric Analysis of Hela cells, using anti-DCK antibody (BD-PE0471), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0471)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0471)

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DCK monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0471)

Flow cytometric Analysis of Jurkat cells, using anti-DCK antibody (BD-PE0471), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCK(Cat# BD-PE0471). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.