BD-PE0461-CYP2E1 (Cytochrome P450 2E1) (2B15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP2E1 (Cytochrome P450 2E1) (2B15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0461

产品名称

CYP2E1 (Cytochrome P450 2E1) (2B15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Cytochrome P450 2E1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP2E1 (NP_000764) produced in HEK293T cell.

稀释度

WB 1:500, IHC 1:50, IF 1:100

预测分子量

56.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0461, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0461, Dilution 1:50)

Immunofluorescent staining of HepG2 cells using anti-CYP2E1 mouse monoclonal antibody (BD-PE0461).

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-CYP2E1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0461, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2E1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2E1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.