BD-PE0458-CYP2B6 (Cytochrome P450 2B6) (7L11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP2B6 (Cytochrome P450 2B6) (7L11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0458

产品名称

CYP2B6 (Cytochrome P450 2B6) (7L11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Cytochrome P450 2B6

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP2B6(NP_000758) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

56.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2B6 antibody (BD-PE0458), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-CYP2B6 antibody (BD-PE0458), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYP2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Figure from citation: Western blot analysis of CYP2B6 protein level by using anti-CYP2B6 antibody in liver samples of patients with obstructive cholestasis. Dilution: 1:2000

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYP2B6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0458)

Anti-CYP2B6 mouse monoclonal antibody (BD-PE0458) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2B6 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2B6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2B6(Cat# BD-PE0458). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.