BD-PE0454-CYP2A6 (Cytochrome P450 2A6) (14M12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP2A6 (Cytochrome P450 2A6) (14M12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0454

产品名称

CYP2A6 (Cytochrome P450 2A6) (14M12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CYP2A6

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP2A6(NP_000753) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

56.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYP2A6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0454)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP2A6 antibody (BD-PE0454), and then analyzed by flow cytometry.

Anti-CYP2A6 mouse monoclonal antibody (BD-PE0454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP2A6 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP2A6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP2A6. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.