BD-PE0453-CYP1A2 (8J16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP1A2 (8J16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0453

产品名称

CYP1A2 (8J16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Cytochrome P450 1A2

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.

稀释度

IHC 1:100

预测分子量

58.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded mouse normal kidney tissue using mouse anti-CYP1A2 05 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE0453 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using mouse anti-CYP1A2 05 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE0453 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-CYP1A2 mouse monoclonal antibody. (BD-PE0453; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-CYP1A2 mouse monoclonal antibody (BD-PE0453, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYP1A2 mouse monoclonal antibody. (BD-PE0453; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CYP1A2 mouse monoclonal antibody. (BD-PE0453; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)