BD-PE0449-CYP1A2 (Cytochrome P450 1A2) (3Y2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP1A2 (Cytochrome P450 1A2) (3Y2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0449

产品名称

CYP1A2 (Cytochrome P450 1A2) (3Y2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CYP1A2

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

58.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CYP1A2 antibody (BD-PE0449), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-CYP1A2 antibody (BD-PE0449), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue N93ing anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Anti-CYP1A2 mouse monoclonal antibody (BD-PE0449) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .

Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-CYP1A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0449, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP1A2(Cat# BD-PE0449). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.