产品名称
NCPR Rabbit Polyclonal Antibody
蛋白名称
NADPH--cytochrome P450 reductase (CPR) (P450R) (EC 1.6.2.4)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P16435
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P16435/entry
Mouse Swissprot No.
P37040
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P37040
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P00388
免疫原
Synthesized peptide derived from part region of human protein
特异性
NCPR Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008],
组织表达
Aorta endothelial cell,Liver,Lung,Small intestine,
细胞定位
Endoplasmic reticulum membrane ; Single-pass membrane protein ; Cytoplasmic side .
功能
catalytic activity:NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein.,cofactor:FAD.,cofactor:FMN.,disease:Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].,disease:Defects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.,function:This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,similarity:In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.,subcellular location:Anchored to the ER membrane by its N-terminal hydrophobic region.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
