BD-PE0437-CD3E (16C4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD3E (16C4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0437

产品名称

CD3E (16C4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD3E

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.

稀释度

WB 1:200~4000, IHC 1:150, IF 1:100

预测分子量

20.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE0437) (1:2000) (1:4000)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0437)

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE0437) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE0437) (1:2000) (1:4000)

Western blot analysis of extracts(50ug) from Jurkat cell lines lysates by using anti-CD3E monoclonal antibody. (BD-PE0437, 1:500)

Anti-CD3E mouse monoclonal antibody (BD-PE0437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE0437) (1:2000) (1:4000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E(Cat# BD-PE0437). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.