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ALDH3A1 (6A4) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0423
产品名称
ALDH3A1 (6A4) Mouse Monoclonal antibody
类别
常规抗体
基因名称
ALDH3A1
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human ALDH3A1(NP_000682) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:50, IF 1:100, FCM 1:100
预测分子量
50.2kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0423, Dilution 1:50)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1(Cat# BD-PE0423). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Anti-ALDH3A1 mouse monoclonal antibody (BD-PE0423) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1 .

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0423, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0423, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0423, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0423, Dilution 1:50)

HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ALDH3A1 mouse monoclonal (BD-PE0423), and then analyzed by flow cytometry.

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