BD-PE0391-CD4 (16P19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD4 (16P19) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE0391

产品名称

CD4 (16P19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.

稀释度

IHC 1:50~100

预测分子量

48.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunofluorescent staining of Jurkat cells using anti-CD4 mouse monoclonal antibody (BD-PE0391, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.

Western Blot analysis of HL-60 and SW-620 cell lysates (35ug) by using anti-CD4 monoclonal antibody

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (BD-PE0391; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD4 4 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0391 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the germinal and non germinal cells have weak to medium cytoplasmic and memeranous staining.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (BD-PE0391; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)