BD-PE0390-CD4 (19P5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD4 (19P5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0390

产品名称

CD4 (19P5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150

预测分子量

48.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-CD4 antibody (BD-PE0390, Red), compared to an IgG isotype control (green), and negative control (PBS, Blue) (1:100).

Flow cytometric Analysis of living human peripheral blood cells, using anti-CD4 antibody (BD-PE0390, A.right, B.red), compared to an IgG isotype control (A.left, B.blue) (1:100).

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (BD-PE0390) Dilution: 1:150

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.