BD-PE0385-CD44 (7S19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD44 (7S19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0385

产品名称

CD44 (7S19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD44

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.

稀释度

WB 1:400~4000, IHC 1:150, IF 1:100

预测分子量

79.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0385)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0385)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD44 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunofluorescent staining of HeLa cells using anti-CD44 mouse monoclonal antibody (BD-PE0385).

Anti-CD44 mouse monoclonal antibody (BD-PE0385) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD44 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD44. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-CD44 monoclonal antibody (1:500).