BD-PE0372-TNF (9E17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TNF (9E17) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE0372

产品名称

TNF (9E17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TNF

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TNF(NP_000585) produced in HEK293T cell.

稀释度

WB 1:4000, IF 1:100

预测分子量

25.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (blue) were immunostained by anti-TNF antibody (BD-PE0372), and then analyzed by flow cytometry (1:100).

Immunofluorescent staining of HeLa cells using anti-TNF mouse monoclonal antibody (BD-PE0372).

Anti-TNF mouse monoclonal antibody (BD-PE0372) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TNF .

Flow cytometric Analysis of Raw264.7 cells, using anti-TNF antibody (BD-PE0372), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNF (Cat# BD-PE0372). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.