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IL1A (2O1) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0360
产品名称
IL1A (2O1) Mouse Monoclonal antibody
类别
常规抗体
基因名称
IL1 alpha
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150, IF 1:100
预测分子量
30.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0360)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0360)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0360)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0360)

Anti-IL1A mouse monoclonal antibody (BD-PE0360) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0360)

HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL1A (NP_000566) produced in HEK293T cell (5 ug, right lane)were separated by SDS-PAGE and immunoblotted with anti-IL1A.

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

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