OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FCGR1A mouse monoclonal antibody (BD-PE0357). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Flow cytometric analysis of living 293T cells transfected with FCGR1A overexpression plasmid , Red)/empty vector (Blue) using anti-FCGR1A antibody (BD-PE0357). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (35ug) from 1 cell line and 1 tissue lysates by using anti-FCGR1A monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD64 (FCGR1A) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0357 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-CD64 (FCGR1A) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0357 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR1A (1:2000).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded mouse brain tissue using anti-CD64 (FCGR1A) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0357 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-CD64 (FCGR1A) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0357 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunofluorescent staining of HeLa cells using anti-FCGR1A mouse monoclonal antibody (BD-PE0357, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-FCGR1A mouse monoclonal antibody. (BD-PE0357; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)