BD-PE0346-VHL (Von Hippel Lindau) (4D1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

VHL (Von Hippel Lindau) (4D1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0346

产品名称

VHL (Von Hippel Lindau) (4D1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Von Hippel Lindau

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human VHL(NP_000542) produced in HEK293T cell.

稀释度

WB 1:4000, IHC 1:150

预测分子量

24kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VHL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VHL. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-VHL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0346)

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (lane 1) , human VHL plasmid (lane 2), mouse VHL plasmid (lane 3) using anti-VHL antibody BD-PE0346 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)