BD-PE0344-Purified P53 (TP53) (2N14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified P53 (TP53) (2N14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0344

产品名称

Purified P53 (TP53) (2N14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

p53

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:50~100

预测分子量

43.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TP53 mouse monoclonal antibody (BD-PE0344). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Western Blot analysis of extracts (35ug) from 11 different cell lines by using anti-TP53 monoclonal antibody

Immunofluorescent staining of HT-29 cells using TP53 mouse monoclonal antibody (BD-PE0344, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunofluorescent staining of COS7 cells using TP53 mouse monoclonal antibody (BD-PE0344, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TP53 mouse monoclonal antibody. (BD-PE0344; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)