BD-PE0338-TP53 (14L18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TP53 (14L18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0338

产品名称

TP53 (14L18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TP53

推荐应用

反应种属

Human,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.

稀释度

WB 1:500~2000, IF 1:100, FCM 1:100

预测分子量

43.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (BD-PE0338), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (BD-PE0338), (Red), compared to a nonspecific negative control antibody, (Blue).

Anti-TP53 mouse monoclonal antibody (BD-PE0338) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53 .

Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (BD-PE0338), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.