Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0335)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1 (Cat# BD-PE0335). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.