BD-PE0296-AK1 (Adenylate Kinase 1) (5C7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AK1 (Adenylate Kinase 1) (5C7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0296

产品名称

AK1 (Adenylate Kinase 1) (5C7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Adenylate Kinase 1

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IF 1:50, FCM 1:100

预测分子量

21.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1(Cat# BD-PE0296). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunofluorescent staining of HT29 cells using anti-AK1 mouse monoclonal antibody (BD-PE0296).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK1 antibody (BD-PE0296), and then analyzed by flow cytometry.