BD-PE0284-PECAM1 (14F1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PECAM1 (14F1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0284

产品名称

PECAM1 (14F1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD31

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500, IHC 1:100

预测分子量

82.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.

Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody (BD-PE0284) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

Western blot analysis of extracts (35ug) from 9 different human tissues by using anti-PECAM1 monoclonal antibody. 1:250

Western blot analysis of extracts (35ug) from Jurkat cell line by using anti-PECAM1 monoclonal antibody. 1:500

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-CD31 (PECAM1) mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0284 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded human placenta using anti-PECAM mouse monoclonal antibody (BD-PE0284) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells and weak staining in the trophoblast.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECAM1 monoclonal antibody at 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-PECAM1mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PECAM1mouse monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1 mouse monoclonal antibody.1:500`

Immunohistochemical staining of paraffin-embedded human skin using anti-PECAM mouse monoclonal antibody (BD-PE0284) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells.

Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-PECAM1mouse monoclonal antibody.

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-PECAM1 monoclonal antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PECAM1mouse monoclonal antibody.