BD-PE0274-L1CAM (3H14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

L1CAM (3H14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0274

产品名称

L1CAM (3H14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

L1CAM

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.

稀释度

IHC 1:100

预测分子量

137.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Confocal immunofluoresce image of primary rat neurons labeled with anti-L1CAM mouse monoclonal antibody (BD-PE0274, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Western blot analysis of extracts (35ug) from 3 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded human brain using anti-L1CAM mouse monoclonal antibody (BD-PE0274) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows positive cytoplasmic and membraneous stain granular layer and molecular layer.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-L1CAM mouse monoclonal antibody (BD-PE0274) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows strong positive membraneous and cytoplasmic staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-L1CAM mouse monoclonal antibody. (BD-PE0274; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-L1CAM mouse monoclonal antibody (BD-PE0274) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows positive cytoplasmic and membraneous stain in a subset of the tumor cells.

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-L1CAM mouse monoclonal antibody (BD-PE0274) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows tumor cells with positive cytoplasmic and membraneous stain.

Immunohistochemical staining of paraffin-embedded human pituitary using anti-L1CAM mouse monoclonal antibody (BD-PE0274) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows only a subset of cells with positive cytoplasmic and membraneous stain.

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-L1CAM mouse monoclonal antibody. (BD-PE0274; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)