OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-L1CAM mouse monoclonal antibody (BD-PE0273). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Confocal immunofluoresce image of primary rat neurons labeled with anti-L1CAM mouse monoclonal antibody (BD-PE0273, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-L1CAM mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0273 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-L1CAM mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0273 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-L1CAM mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0273 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded human brain using anti-L1CAM mouse monoclonal antibody (BD-PE0273) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining seen in staining in granular layer and the molecular layer.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-L1CAM mouse monoclonal antibody (BD-PE0273) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining seen in staining in tumor cells.

Immunohistochemical staining of paraffin-embedded mouse brain tissue using anti-L1CAM mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE0273 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot of cell lysates (35ug) from HeLa. Diluation: 1:500

Immunohistochemical staining of paraffin-embedded human pituitary using anti-L1CAM mouse monoclonal antibody (BD-PE0273) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL HIER buffer using pressure chamber for 3 minutes at 110C. Image shows only a subset of cells with positive cytoplasmic and membraneous stain.

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-L1CAM mouse monoclonal antibody. (BD-PE0273; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human pancreas using anti-L1CAM mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE0273); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen glandular cells.

Immunohistochemical staining of paraffin-embedded human ovcarian carcinoma using anti-L1CAM mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE0273); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Cytoplasmic and membraneous staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-L1CAM mouse monoclonal antibody. (BD-PE0273; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)