BD-PE0271-L1CAM (10G5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

L1CAM (10G5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0271

产品名称

L1CAM (10G5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

L1CAM

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.

稀释度

WB 1:500~2000, FCM 1:100

预测分子量

137.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (BD-PE0271), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-L1CAM antibody (BD-PE0271), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.