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L1CAM (10W1) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0269
产品名称
L1CAM (10W1) Mouse Monoclonal antibody
类别
常规抗体
基因名称
L1CAM
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
稀释度
WB 1:1000, IHC 1:50, IF 1:100, FCM 1:100, IP 2-4ug/mg
预测分子量
140.0kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of Hela cells, using anti-L1CAM antibody (BD-PE0269), (Red) compared to a nonspecific negative control antibody (Blue).

Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody (BD-PE0269, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Flow cytometric analysis of Jurkat cells, using anti-L1CAM antibody (BD-PE0269), (Red) compared to a nonspecific negative control antibody (Blue).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-L1CAMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0269, Dilution 1:50)

Anti-L1CAM mouse monoclonal antibody (BD-PE0269) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM (Cat# BD-PE0269). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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