BD-PE0265-KRT5 (Keratin 5) (1F14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KRT5 (Keratin 5) (1F14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0265

产品名称

KRT5 (Keratin 5) (1F14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT5

推荐应用

反应种属

Human,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Synthetic peptide corresponding to the conserved region of human KRT5 and KRT6

稀释度

WB 1:500, IHC 1:150

预测分子量

62.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0265)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0265)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-KRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0265)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE0265) (1:1000)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (BD-PE0265) (1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT5(Cat# BD-PE0265). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.