BD-PE0262-IL2RA (9I2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IL2RA (9I2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0262

产品名称

IL2RA (9I2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IL2RA

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IL2RA (NP_000408) produced in HEK293T cell.

稀释度

WB 1:1000, FCM 1:100

预测分子量

30.82kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL2RA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL2RA antibody (Cat# BD-PE0262)(1:1000)

Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-IL2RA antibody (BD-PE0262).(1:100).

Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h using anti-IL2RA antibody (BD-PE0262)(Right). Cells incubated with a non-specific antibody (Left) were used as isotype control.(1:100).

Flow cytometric analysis of living 293T cells transfected with IL2RA overexpression plasmid , Red)/empty vector (Blue) using anti-IL2RA antibody (BD-PE0262). Cells incubated with a non-specific antibody (Green) were used as isotype control. （1：100）