BD-PE0255-GALE (7A9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GALE (7A9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0255

产品名称

GALE (7A9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GALE

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GALE (NP_000394) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150

预测分子量

38.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GALE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-GALE monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GALE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0255)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALE(Cat# BD-PE0255). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.