BD-PE0249-CYB5R3 (17R5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYB5R3 (17R5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0249

产品名称

CYB5R3 (17R5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CYB5R3

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYV5R3(NP_000389) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100

预测分子量

34.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

Anti-CYB5R3 mouse monoclonal antibody (BD-PE0249) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYB5R3 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0249)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.