BD-PE0232-Troponin T (TNNT2) (5N8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Troponin T (TNNT2) (5N8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0232

产品名称

Troponin T (TNNT2) (5N8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TNNT2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TNNT2 (NP_000355) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

35.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. (provided by RefSeq, Jul 2008)

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNNT2 mouse monoclonal antibody (BD-PE0232). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Western blot analysis of extracts (25ug) from 4 different cell lines by using anti-TNNT2 monoclonal antibody. (1:500) (1: HEK293; 2: Hela; 3: MCF7; 4: HepG2) Dilution: 1:500

Western blot of human tissue lysates (15ug) from 2 different tissues (1: Heart, 2: Muscle). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, BD-PE0232) (1:200)