BD-PE0227-TNNI3 (Cardiac Troponin I) (12U6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TNNI3 (Cardiac Troponin I) (12U6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0227

产品名称

TNNI3 (Cardiac Troponin I) (12U6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

TNNI3

推荐应用

反应种属

Human,Dog,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human TNNI3 (NP_000354) produced in E.coli.

稀释度

WB 1:2500~5000, IF 1:50~100

预测分子量

23.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody (BD-PE0227).

Immunofluorescent staining of HeLa cells using anti-TNNI3 mouse monoclonal antibody (BD-PE0227).

Anti-TNNI3 mouse monoclonal antibody (BD-PE0227) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TNNI3 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.