BD-PE0220-SOX9 (3M7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SOX9 (3M7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0220

产品名称

SOX9 (3M7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SOX9

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1-205 of human SOX9 (NP_000337) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

56kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of HT29 cell lysate (35ug) by using anti-SOX9 monoclonal antibody. Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, BD-PE0220)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, BD-PE0220)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, BD-PE0220)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SOX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, BD-PE0220)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX9(Cat# BD-PE0220). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.