BD-PE0212-RB1 (2R10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RB1 (2R10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0212

产品名称

RB1 (2R10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli.

稀释度

WB 1:2000, IHC 1:500

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RB1 monoclonal antibody. 1:400

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0212) (1:500)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0212) (1:500)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0212) (1:500)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0212) (1:500)

Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0212) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RB1 mouse monoclonal antibody.1:500`