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SERPINA1 (14V16) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0195
产品名称
SERPINA1 (14V16) Mouse Monoclonal antibody
类别
常规抗体
基因名称
SERPINA1
推荐应用
WB
反应种属
Human,Dog,Monkey
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector
稀释度
WB 1:500~1000, IHC 1:150, FCM 1:100, IP 2ug/500ul
预测分子量
46.7kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase,plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0195)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-SERPINA1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0195)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0195)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0195)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0195)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1(Cat# BD-PE0195). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (BD-PE0195), and then analyzed by flow cytometry.

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