BD-PE0192-SERPINA1 (17F8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SERPINA1 (17F8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0192

产品名称

SERPINA1 (17F8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SERPINA1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 26-418 of human A1AT (NP_000286) produced in E.coli.

稀释度

WB 1:500~1000, IHC 1:50, FCM 1:100

预测分子量

46.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

A1AT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in A1AT gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (BD-PE0192), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0192)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.