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PGAM2 (4C15) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0188
产品名称
PGAM2 (4C15) Mouse Monoclonal antibody
类别
常规抗体
基因名称
PGAM2
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human PGAM2(NP_000281) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150, IF 1:100
预测分子量
28.6kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0188)

Anti-PGAM2 mouse monoclonal antibody (BD-PE0188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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