BD-PE0185-PEPD (12O16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PEPD (12O16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0185

产品名称

PEPD (12O16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PEPD

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

54.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PEPD antibody (BD-PE0185), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PEPD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

Anti-PEPD mouse monoclonal antibody (BD-PE0185) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PEPD .

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0185, Dilution 1:50)

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PEPD monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.