BD-PE0162-MET (10I16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MET (10I16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0162

产品名称

MET (10I16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MET

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 570-885 of human MET(NP_000236) produced in E.coli.

稀释度

IHC 1:150

预测分子量

153kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq, Jul 2008)

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded human placenta using anti-MET 90 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0162 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the trophoblasts.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-MET 90 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0162 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in epithelial cells.

Western blot of whole cell lysate (25ug) (1: MCF7; 2: HeLa; 3: Jurkat; 4: PC-12; 5: COS-7; 6: A549). The labelled upper band is Pro-Met and the labelled lower band is Met. Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE0162) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE0162) (1:150)

Immunohistochemical staining of paraffin-embedded human adjacent normal lung using anti-MET 90 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0162 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic in brochial tubes.

Immunofluorescent staining of HeLa cells using anti-MET mouse monoclonal antibody (BD-PE0162, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE0162) (1:150)

Immunohistochemical staining of paraffin-embedded human renal carcinoma using anti-MET 90 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE0162 requires heat-induced epitope retrieval with ACCEL in a presure cooker for 3 minutes at 110C. The image shows strong membranous and cytoplasmic staining in the tumor cells of the kidney.