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KRT18 (3V7) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0147
产品名称
KRT18 (3V7) Mouse Monoclonal antibody
类别
常规抗体
基因名称
KRT18
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 69-372 of human KRT18(NP_000215) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
预测分子量
47.9kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of permeabilized MCF-7 cells, using anti-KRT18 antibody (BD-PE0147), (Red), compared to negative control (PBS), (Blue) (1:100).

Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-KRT18 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KRT18 mouse monoclonal antibody.This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE0147) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18 (Cat# BD-PE0147)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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